Preparation of rat spleen for functional analysis

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Abstract

Spleen cell Separation from JPR on Vimeo.

This material was designed to provide a detailed review of the preparation of spleen cells for immunophenotyping. Speen must be reduced to a single cell suspension before cells can be stained with monoclonal antibodies and then run on the flow cytometer for determination of T cell, B cell subsets as well as other cell enumeration procedures.

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Researchers should cite this work as follows:

  • Joseph Paul Robinson (2017), "Preparation of rat spleen for functional analysis," https://sparc.purdue.edu/resources/55.

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