J. Paul Robinson

J. Paul Robinson

Address:
J. Paul Robinson, Ph.D.
The SVM Professor of Cytomics
& Professor of Biomedical Engineering
Lynn Hall, G221
West Lafayette, IN 47907
tel.: (765) 494-0757
e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it.
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Paul Robinson

J. Paul Robinson is the SVM Professor of Cytomics in the College of Veterinary Medicine and a professor of biomedical engineering in the Weldon School of Biomedical Engineering at Purdue University. He received his Ph.D. in Immunopathology from the University of New South Wales, Sydney, Australia. He completed a postdoctoral fellowship at the University of Michigan Medical School. He is currently the director of the Purdue University Cytometry Laboratories at Purdue University. His research area has been focused on reactive oxygen species primarily in neutrophils and cell lines such as HL-60 cells. His lab is currently focused on mitochondrial function. In addition he is engaged in developing translational tools for better diagnostics for cervical cancer. Over the past several years, his group has expanded their interest in bioengineering with hardware and software groups developing innovative technologies such as hyperspectral cytometry using multiarray PMTs (currently commercialized by Sony), optical tools for quantitative fluorescence measurement and advanced classification approaches for clinical diagnostics and bacterial classification, and high content, high throughput screening technologies.

Robinson's Research

Research agenda

My background is in immunopathology, and I have had a long-term interest in evaluation of phenotypic analysis of blood cells. My interests for many years have been in the role of phagocytic cells in the inflammatory response. In particular, we have developed several cell analysis technologies for functional analysis of white blood cells using flow cytometry and various imaging modalities. In order to evaluate drug toxicity, we have developed functional, high-throughput, cellular screens, such as mitochondrial function, glutathione, viability etc. Recently, we have been evaluating cytokine status of rats undergoing various stress environments. In addition, we have significant experience in evaluating phenotypic status of blood samples, particularly the dissection of T cell, B cell and a variety of other cellular subsets. We also have a lot of experience in sorting cell populations for future cell culturing needs, or for additional molecular or genetic analysis. We have access to multiple technologies for cellular and molecular characterization in our lab, including single cell analysis tools such as flow cytometry and advanced image analysis systems. These activities will directly support this application.

Selected publications

  • Tsiper, Maria V.; Sturgis, Jennifer; Avramova, Larisa V.;Parakh, Sl, Fatig, R, Juan-Garcia, A, Li, NY. Rajwa, B; Narayanan, P; Qualls, CW, Robinson, JP, Davisson, VJ. Differential Mitochondrial Toxicity Screening and Multi-Parametric Data Analysis; PLOS ONE 7(10): e45226, DOI: 10.1371/journal.pone.0045226
  • Yiqing Lu1, Jie Lu, Jiangbo Zhao, Janet Cusido, Françisco M. Raymo, Jingli Yuan, Sean Yang, Robert C. Leif, Yujing Huo, James A. Piper, J. Paul Robinson, Ewa M. Goldys, and Dayong Jin. On-the-fly decoding luminescence lifetimes in the microsecond region for lanthanide-encoded suspension arrays; Nature Biophotonics,DOI: 10.1038/ncomms4741
  • Ran An, Dan Merrill, Larisa Avramova, Jennifer Sturgis, Maria Tsiper, J Paul Robinson, John Turek, David D Nolte; Phenotypic Profiling of Raf Inhibitors and Mitochondrial Toxicity in 3D Tissue Using Biodynamic Imaging, Journal of Biomolecular Screening 12/2013, DOI:10.1177/1087057113516674
  • J. Paul Robinson, Valery Patsekin, Cheryl Holdman, Kathy Ragheb, Jennifer Sturgis, Ray Fatig, Larisa V. Avramova, Bartek Rajwa, V. Jo Davisson, Nicole Lewis, Padma Narayanan, Nianyu Li, C.J. Qualls High-throughput secondary screening at the single-cell level; Journal of Laboratory Automation, 18:85-98, 2013 JALA, DOI:10.1177/22110682/2456978
  • Gregori, G., Patsekin, V., Rajwa, B., Jones, J., Ragheb, K., Holdman, C., Robinson, J. Paul, Hyperspectral cytometry at the single cell level using a 32 channel photodetector. Cytometry, Volume 81A(1):35–44, 2012, PMID:22173900, DOI: 10.1002/cyto.a.21120